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  • Title: Quantitative competitive polymerase chain reaction: analysis of amplified products of the HIV-1 gag gene by capillary electrophoresis with laser-induced fluorescence detection.
    Author: Williams SJ, Schwer C, Krishnarao AS, Heid C, Karger BL, Williams PM.
    Journal: Anal Biochem; 1996 Apr 05; 236(1):146-52. PubMed ID: 8619480.
    Abstract:
    A quantitative competitive polymerase chain reaction (PCR) assay for the detection and quantification of HIV-1 has been developed using capillary electrophoresis. Separation of the PCR products is carried out in coated capillaries filled with replaceable linear polyacrylamide, and detection is performed using laser-induced fluorescence. The quantitative capabilities of this assay are described. We show results from analysis of a noninfectious plasmid encoding the HIV genome and integrated proviral DNA. Potential applications of this assay are discussed.
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