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  • Title: In-situ-isolated perfused rat pancreas: a new method for pharmacological studies of the exocrine pancreas.
    Author: Tournut R, Estival A, Vaysse N, Pascal JP, Ribet A.
    Journal: Digestion; 1977; 15(4):329-37. PubMed ID: 863135.
    Abstract:
    A method of in-situ-isolated perfused rat pancreas is described. In situ perfusion of the isolated rat pancreas allows a faster set-up, thanks to a simpler operating procedure. The sensitivity of the isolated rat pancreas has been tested with secretin and cholecystokinin. The pancreas responds to doses of secretin as low as 0.005 CU/kg-h. Such a highly sensitive model might prove to be useful for the bioassay of secretin and the study of the effects of pancreatic secretagogues.
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