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  • Title: Electron microscopic data for the presence of post-meiotic gene expression in isolated ram sperm chromatin.
    Author: Miteva K, Valkov N, Goncharova-Peinoval J, Kovachev K, Zlatarev S, Pironcheva G, Russev G.
    Journal: Cytobios; 1995; 83(333):85-90. PubMed ID: 8635320.
    Abstract:
    A whole mount electron microscopic technique facilitated a direct visualization of transcripts in ram spermatozoa in run-on experiments. The localization of transcripts in sperm chromatin by spreading enabled identification of regions where transcription complexes, presumably pre-mRNA species, were seen related to chromatin. In another series of experiments the localization of polymerase II was demonstrated using a specific antibody against the conservative tail domain of the polymerase II molecule, followed by protein A-gold visualization on spread chromatin and on thin sections from mature spermatozoa. Incorporation of bio-UTP in transcripts was visualized by streptavidin-gold on spreading and on thin sections. The data suggest that transcription occurs at the periphery of the mature spermatozoa.
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