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  • Title: Molecular sizes and relationships of TOL plasmids in Pseudomonas.
    Author: Duggleby CJ, Bayley SA, Worsey MJ, Williams PA, Broda P.
    Journal: J Bacteriol; 1977 Jun; 130(3):1274-80. PubMed ID: 863855.
    Abstract:
    Plasmid deoxyribonucleic acid was isolated from thirteen Pseudomonas strains judged on genetic criteria to carry plasmids coding for the degradation of toluene and m- and p-xylenes (TOL plasmids). Most strains carried a single species, but two strains carried two size classes, and cells of a third strain contained plasmids ranging in size from 25 X 10(6) to 202 X 10(6) daltons. Some plasmids could be transformed into a Pseudomonas putida strain to yield Tol+ progeny. Plasmids from 5 of the 13 strains were indistinguishable on the basis of size and gel pattern of fragments after endonuclease digestion.
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