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  • Title: Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II.
    Author: Ito T, Xu Q, Takeuchi H, Kubo T, Natori S.
    Journal: FEBS Lett; 1996 Apr 29; 385(1-2):21-4. PubMed ID: 8641458.
    Abstract:
    Previously, we characterized a rat cDNA for testis-specific transcription elongation factor S-II (SII-T1) (Q. Xu et al., J. Biol. Chem. 269, 3100-3103 (1994)). Here, we isolated a 335-bp fragment of the cDNA for mouse SII-T1, and used it to examine the expression of the SII-T1 gene in the testis by in situ hybridization. The results indicated that the SII-T1 gene is expressed exclusively in spermatocytes, showing no appreciable expression in spermatogonia, spermatids, or Leydig cells. RT-PCR experiments using testis RNA from W/Wv mutant mice also suggested that SII-T1 is a specific transcription elongation factor essential for spermatogenesis.
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