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  • Title: Use of peptide immunization for porcine insulin of a very high homology with a host protein.
    Author: Katoh S, Terashima M, Tomioka K, Hikida N.
    Journal: J Immunol Methods; 1996 May 10; 191(1):33-8. PubMed ID: 8642198.
    Abstract:
    An anti-peptide antibody was obtained against a peptide corresponding to the 12 amino acids of the C-terminal region of porcine insulin B chain. The adsorption characteristics of this antibody were compared with those of anti-porcine insulin and anti-sheep insulin antibodies. Immunization of rabbits using the peptide corresponding to the C-terminal region of porcine insulin B chain produced an anti-peptide antibody which reacted with native porcine insulin with a much higher association constant than that of an anti-porcine insulin antibody. In addition, this immunization technique did not cause any observable physiologically harmful effects, such as hypoglycemia, in the immunized rabbits. Thus, peptide immunization may be a useful strategy when target proteins have biological activity and/or toxicity, and also have a very high degree of homology with the corresponding proteins of the immunized animal, which may inhibit the production of antibodies with a high association constant.
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