These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Impairment of DNA replication within one cell cycle after seeding of cells in the presence of a polyamine-biosynthesis inhibitor.
    Author: Fredlund JO, Oredsson SM.
    Journal: Eur J Biochem; 1996 May 01; 237(3):539-44. PubMed ID: 8647096.
    Abstract:
    Chinese hamster ovary (CHO) cells in the plateau phase were seeded in the absence or presence of 5 mM 2-difluoromethylornithine (F2MeOrn), an enzyme-activated irreversible inhibitor of ornithine decarboxylase. The thymidine analogue bromodeoxyuridine (BrdUrd, 5 microM) was added to the culture medium 30 min before sampling of the cells, which occurred 1-17 h after seeding. Using flow cytometry, coupled with an indirect immunofluorescence technique, which utilized monoclonal BrdUrd and secondary fluorescein-isothiocyanate-conjugated antibodies, and the DNA stain propidium iodide, cellular BrdUrd and DNA contents were quantified. To determine if there was a perturbation in the progression of cells through the S phase, the distribution of BrdUrd-labelled cells in the S phase was evaluated in two ways: (a) by calculating the mean DNA content of BrdUrd-labelled cells in relation to the mean DNA contents of G1 and G2 cells (relative movementzero) and (b) by studying DNA histograms of BrdUrd-labelled cells. By using both evaluation methods, we show that DNA replication was impaired during the first cell cycle that was initiated after seeding CHO cells in the presence of F2MeOrn. The cells appeared to enter the S phase normally but were then delayed in their progression through this phase. The impairment of F2MeOrn treatment on DNA replication was apparent at 9 h after seeding, a time point at which the putrescine pool was depleted, the spermidine pool was approximately halved, and the spermine pool was unaffected, when compared to corresponding pools of control cells. When cells were seeded in the presence of F2MeOrn and putrescine, the effect on DNA replication was prevented. The rates of incorporation of [3H]uridine and [3H]leucine into RNA and protein, respectively, were the same in control and in F2MeOrn-treated cells for at least up to 11 h after seeding.
    [Abstract] [Full Text] [Related] [New Search]