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  • Title: [Introduction of mutator phage D3112 of Pseudomonas aeruginosa into Alcaligenes eutrophus var. metallotollerans (Strain CH34)].
    Author: Krylov VN.
    Journal: Genetika; 1996 Jan; 32(1):146-9. PubMed ID: 8647419.
    Abstract:
    It is demonstrated that the intact genome of a D3112 tranposable phage (TP) of Pseudomonas aeruginosa, integrated into a recombinant plasmid RP4 :: D3112, can be transferred by means of conjugation from P. putida PpG1 (RP4npt :: D3112) donor cells into Alcaligenes eutrophus var. metallotollerans cells. P. aeruginosa strains are unacceptable as donors because they have a bactericidal effect on A. eutrophus. RP4npt :: D3112 plasmid is stably inherited by A. eutrophus with D3112 being expressed and successfully reproduced. However, TP loses the induction ability after UV irradiation or mitomycin C treatment. It is suggested that D3112 TP and its miniderivatives could be used in manipulations with A. eutrophus var. metallotolerans.
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