These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Relationship of protein synthesis to mRNA levels in the liver of chicks under various nutritional conditions.
    Author: Kita K, Matsunami S, Okumura J.
    Journal: J Nutr; 1996 Jun; 126(6):1610-7. PubMed ID: 8648435.
    Abstract:
    The rate of liver protein synthesis in vivo and hepatic messenger ribonucleic acid (mRNA) levels were measured to examine the mechanisms regulating liver protein synthesis in chicks under various nutritional conditions. Fractional synthesis rate (FSR) of liver protein was measured by using a large dose injection of L-[4-3H]phenylalanine. Poly(A)+RNA was extracted as mRNA from total RNA using poly(U)-Sepharose 4B affinity chromatography. The influence of varying dietary protein levels (0-60%) on protein synthesis and mRNA concentration in the liver of chicks was examined. Both FSR (%/d) and absolute synthesis rate (ASR, mg/d) of liver protein increased with increasing dietary protein levels from 0 to 20% (the dietary protein requirement), whereas when dietary protein increased from 20 to 40%, both rates decreased significantly. This change was accounted for by changes in RNA concentration (RNA:protein ratio) and hepatic mRNA content. Second, the time course of changes in liver protein synthesis and hepatic mRNA content were measured in force-fed chicks that had been deprived of food for 2 d. Liver protein synthesis (FSR) was significantly greater 30 min after refeeding compared with that of unfed chicks. Liver protein synthesis (FSR) correlated with the activity of RNA to synthesize protein (r = 0.61, P < 0.001), and ASR was correlated with hepatic mRNA content (r = 0.37, P = 0.030). Third, experimental diets containing individual nutrients were force-fed to chicks after 2 d of food deprivation. When chicks were refed carbohydrate and protein diets, liver protein synthesis (FSR) tended to be greater by 24% (P = 0.063) and 22% (P - 0.075) at 30 min after refeeding compared with that of unfed chicks, respectively. These findings indicate that changes in the rate of liver protein synthesis in vivo due to the alteration in nutritional conditions are, at least partially, accounted for by not only RNA concentration and the activity of RNA synthesizing protein but also hepatic mRNA levels.
    [Abstract] [Full Text] [Related] [New Search]