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  • Title: The two major sites of cbl tyrosine phosphorylation in abl-transformed cells select the crkL SH2 domain.
    Author: Andoniou CE, Thien CB, Langdon WY.
    Journal: Oncogene; 1996 May 02; 12(9):1981-9. PubMed ID: 8649859.
    Abstract:
    We recently found that the 120-kD protein product of the c-cbl oncogene is tyrosine phosphorylated in tumor cells generated by bcr-abl or v-abl and that p120cbl will associate with these proteins in vivo. We also found an oncogenic form of cbl protein in the 70Z/3 pre-B cell lymphoma which exhibits deregulated tyrosine phosphorylation. These findings have led us to broaden our study of cbl's involvement in abl-mediated tumorigenesis. Here we show by immunodepletion that cbl is the major 120-kD tyrosine phosphorylated protein in cells which express activated forms of the abl oncogene. We also demonstrate that tyrosine phosphorylation of pl20cbl in bcr-abl transformed cells does not alter its subcellular localization. In addition we show that the oncogenic 7OZ/3 form of cbl exhibits enhanced tyrosine phosphorylation in v-abl infected cells and that cbl is heavily tyrosine phosphorylated in hemopoietic cells transformed by v-src. Finally this study identifies two sites that are essential for the tyrosine phosphorylation of cbl in abl-transformed cells. These sites conform to the preferred abl kinase substrate sequence of YXXP and we show that following phosphorylation they mediate an association with the crkL SH2 domain.
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