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  • Title: Kinetic and spectroscopic characterization of fluorescent ribose-modified ATP analogs upon interaction with skeletal muscle myosin subfragment 1.
    Author: Conibear PB, Jeffreys DS, Seehra CK, Eaton RJ, Bagshaw CR.
    Journal: Biochemistry; 1996 Feb 20; 35(7):2299-308. PubMed ID: 8652570.
    Abstract:
    The interaction of fluorescent ATP analog 2'(3')-O-[N-[2-[3-(5-fluoresceinyl)thioureido]-ethyl]carbamoyl]adenosine 5'-triphosphate (FEDA-ATP) with rabbit skeletal myosin subfragment 1 (S1) and acto-S1 was studied. This and related ATP analogs are potentially useful for determination of the ATPase activity of single myosin filaments using fluorescence microscopy [Sowerby et al. (1993) J. Mol. Biol. 234, 114-123]. However, it is necessary that such analogs mimic ATP in their kinetics of turnover. The apparent second-order association rate constants for FEDA-ATP binding to S1 and for FEDA-ATP-induced dissociation of acto-S1 are about 4 times slower than those for ATP. As with ATP, the hydrolysis step is fast, so that the M.FEDA-ADP.P(i) complex is the major steady-state intermediate. The turnover rate is the same for the 2' and 3' FEDA-ATP derivatives and similar to that of ATP itself. The dissociation rate constant for FEDA-ADP from S1 is identical to that for ADP. Actin-activated turnover is comparable for both FEDA-ATP and ATP. The corresponding rhodamine and sulfoindocyanine, Cy3.18 (Cy3), derivatives also appear to be reasonable analogs. FEDA-ATP binding leads to a 25-40% reduction in fluorescein fluorescence. Spectral properties of the bound nucleotide were explored by trapping FEDA-ADP as its aluminum fluoride complex. The fluorescence quenching is a consequence of a reduction in both absorbance and excited-state lifetime, but there is little change in spectral shape.
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