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  • Title: Heat-labile uracil-DNA glycosylase: purification and characterization.
    Author: Sobek H, Schmidt M, Frey B, Kaluza K.
    Journal: FEBS Lett; 1996 Jun 10; 388(1):1-4. PubMed ID: 8654578.
    Abstract:
    A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40 degrees C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.
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