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  • Title: Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3.
    Author: Sayers TJ, Lloyd AR, McVicar DW, O'Connor MD, Kelly JM, Carter CR, Wiltrout TA, Wiltrout RH, Smyth MJ.
    Journal: J Leukoc Biol; 1996 May; 59(5):763-8. PubMed ID: 8656064.
    Abstract:
    Cytotoxic lymphocytes possess a number of serine proteases (granzymes) usually localized in cytoplasmic granules. To date, the DNA sequences of four human granzymes have been reported. A fifth human granzyme (granzyme 3) has been biochemically purified and its N-terminal amino acid sequence has been reported. This enzyme was described as possessing tryptase activity, cleaving synthetic substrates after arginine or lysine. We recently cloned a rat granzyme tryptase (RNK-Tryp-2), and used this cDNA to screen human cDNA libraries. Isolation of cDNA fragments of a human gene could be overlapped to provide a complete cDNA sequence, which we designated HNK-Tryp-2. The N-terminal amino acid sequence deduced from HNK-Tryp-2 was identical to that reported for granzyme 3. This gene appears to be a single copy gene that is expressed in isolated natural killer cells and T cells as well as in tissues containing these cells.
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