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  • Title: 5-Nitrosothio-2-nitrobenzoate: a reagent for the nitrosation of thiol groups in proteins.
    Author: Studebaker MS, Zhang H, Means GE.
    Journal: Anal Biochem; 1996 Jun 01; 237(2):193-7. PubMed ID: 8660565.
    Abstract:
    The S-nitroso derivative of 5-thio-2-nitrobenzoate was synthesized from 5,5'-dithiobis(2-nitrobenzoic acid) and partially characterized. Although relatively unstable, it is easy to prepare and reacts very rapidly with thiols and thiol groups of proteins to give corresponding S-nitrosothiols and 5-thio-2-nitrobenzoate dianion. The latter's easy spectrophotometric detection makes such reactions easy to follow and to quantitate.
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