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  • Title: A human chromosome 22 fosmid resource: mapping and analysis of 96 clones.
    Author: Birren BW, Tachi-iri Y, Kim UJ, Nguyen M, Shizuya H, Korenberg JR, Simon MI.
    Journal: Genomics; 1996 May 15; 34(1):97-106. PubMed ID: 8661029.
    Abstract:
    We have created a resource for chromosome 22 consisting of 96 unique, well-characterized Fosmids. The Fosmid vector permits efficient cloning of DNA fragments averaging 40 kb in a single-copy vector based on the F factor of Escherichia coli. We have found that Fosmid clones from human chromosome 22 show remarkable stability and are useful for a wide variety of applications in genome analysis. These 96 clones have been localized by FISH, using high-resolution fluorescent banding and multicolor mapping techniques, and their position on the chromosome was correlated with their content of a number of common repeated sequence elements. We identified a subset of clones likely to contain genes by restriction analysis using the enzymes NotI, MluI, SacII, and BssHII. This collection of cytogenetically anchored clones, representing nearly 7% of the chromosome, is of immediate value for detecting chromosomal rearrangements, for use in gene isolation, and as a framework for physical mapping.
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