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  • Title: Differentiation of Borrelia burgdorferi sensu lato strains isolated from skin biopsies and tick by pulsed-field gel electrophoresis.
    Author: Busch U, Hizo Teufel C, Boehmer R, Preacmursic V.
    Journal: Rocz Akad Med Bialymst; 1996; 41(1):51-8. PubMed ID: 8673805.
    Abstract:
    Fifteen isolates of Borrelia burgdorferi sensu lato from skin biopsies (8) and ticks (7) were investigated for species differentiation by pulsed-field gel electrophoresis (PFGE). The skin isolates were further characterized by plasmid profile as well as SDS-PAGE and revealed six Borrelia afzelii-, one Borrelia burgdorferi sensu stricto-, and one Borrelia garinii species. The tick isolates showed three Borrelia afzelii--and four Borrelia garinii species. The skin isolates could be further discriminated by plasmid profile analysis--all strains showed an individual pattern. The SDS-PAGE of whole-cell lysates obtained from skin isolate showed a different protein pattern for each of the skin isolates.
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