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  • Title: Characterization of C-4-methylated sterols by pyridine-induced solvent shifts in proton magnetic resonance spectroscopy.
    Author: Iida T, Tamura T, Miura N, Matsumoto T.
    Journal: Steroids; 1977 Apr; 29(4):453-60. PubMed ID: 867444.
    Abstract:
    The proton magnetic resonance (PMR) spectra were measured in deuterochloroform (CDC13) and pyridine solutions for some 4-desmethyl, 4alpha-methyl, 4beta-methyl, and 4,4-dimethyl sterols related to 5alpha-cholestane series as well as for their C-3-oxo derivatives. The influence of pyridine, relative to CDC13, on methyl group chemical shifts was discusssed. The technique utilizing pyridine-induced solvent shifts in PMR spectroscopy was found useful in characterizing the individual classes of sterols.
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