These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Characterization of the Pacific salmon gonadotropin-releasing hormone gene, copy number and transcription start site.
    Author: Coe IR, von Schalburg KR, Sherwood NM.
    Journal: Mol Cell Endocrinol; 1995 Nov 30; 115(1):113-22. PubMed ID: 8674859.
    Abstract:
    Multiple forms of gonadotropin-releasing hormone (GnRH) have been shown to exist in all vertebrates examined except recently-evolved placental mammals. To study the origin and regulation of the GnRH genes in a Pacific salmon (Oncorhynchus nerka), we isolated and sequenced the salmon form of GnRH. The Southern blot shows a single band that strongly hybridizes to a probe for the gene reported here and weaker bands that may represent genes for related forms of GnRH. There is strong conservation of sequence in the hormone coding region and of the gene organization between fish and mammals. However, the GnRH-associated peptide (GAP) shows very little sequence identity with the mammalian GAPs, questioning its physiological role. We also show for the first time the transcriptional start site for a GnRH gene in a non-mammalian species. Interestingly, a large segment of 1152 nucleotides in the promoter region of the Pacific salmon GnRH gene is missing compared with the Atlantic salmon (Salmo salar) gene. These gene rearrangements suggest that these two salmonid species, which have been geographically separated for 8-15 million years, have evolved promoters with different mechanisms for control and transcription of GnRH.
    [Abstract] [Full Text] [Related] [New Search]