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  • Title: Effect of hyperbaric oxygen on neutrophil concentration and pulmonary sequestration in reperfusion injury.
    Author: Zamboni WA, Wong HP, Stephenson LL.
    Journal: Arch Surg; 1996 Jul; 131(7):756-60. PubMed ID: 8678778.
    Abstract:
    OBJECTIVE: To evaluate the effect of hyperbaric oxygen (HBO) on muscle flap arterial neutrophil concentration and pulmonary neutrophil sequestration following ischemia-reperfusion injury in a rat gracilis muscle microcirculation model. DESIGN: Prospective randomized experimental trials. SETTING: Laboratory. MATERIALS: Male Wistar rats weighing 150 to 300 g. INTERVENTIONS: Gracilis muscle flaps were raised on isolated vascular pedicles. Three groups were evaluated: (1) sham (flap raised, 4-hour observation, no ischemia, no HBO), (2) ischemia control (4-hour flap is ischemia, no HBO); and (3) ischemia (4-hour flap is ischemia) plus HBO (last 90 minutes of ischemia, 100% oxygen, 2.5 atm absolute). MAIN OUTCOME MEASURES: Flap pedicle arterial leukocyte and neutrophil concentrations were assessed at 5 and 90 minutes of reperfusion in the 3 groups. Pulmonary neutrophil sequestration was measured in a blinded fashion, using histologic examination and myeloperoxidase assay in the 3 groups at 5 and 90 minutes of reperfusion. RESULTS: Ischemia produced an increase in pedicle arterial leukocyte and neutrophil concentrations in blood flowing to the gracilis muscle flap; administration of HBO significantly reduced concentration to sham levels (P < .05). There were no significant differences in pulmonary neutrophil sequestration among the experimental groups. CONCLUSIONS: In this rat gracilis muscle microcirculation model, the increase in pedicle arterial leukocyte and neutrophil concentrations following ischemia-reperfusion injury was significantly reduced to sham levels by HBO treatment. This observed reduction was not attributable to HBO-induced pulmonary sequestration, which did not significantly change with HBO administration. Further investigation is required to elucidate the mechanisms of action of HBO in ameliorating ischemia-reperfusion injury in this model.
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