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  • Title: Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli.
    Author: Jin C, Liu H, Yang S, Zhang S.
    Journal: Chin J Biotechnol; 1995; 11(3):185-91. PubMed ID: 8679935.
    Abstract:
    Thermostable DNA polymerase genes had been amplified from Thermus aquaticus YT-1 using the PCR technique. The amplified 2.5-kb DNA fragment was inserted into pUC18 and confirmed to be a thermostable DNA polymerase gene by restriction mapping and DNA sequencing. The insert fragment was then combined into an expression vector, pBV221. This recombinant plasmid overexpressed a 94-kDa of recombinant protein in E. coli. A 100-mL E. coli. culture could yield 1.5 x 10(5) units of Taq DNA polymerase, which could be applied in the PCR.
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