These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A rapid and convenient method to prepare DIG-labelled RNA probes for use in non-radioactive in situ hybridization.
    Author: Gandrillon O, Solari F, Legrand C, Jurdic P, Samarut J.
    Journal: Mol Cell Probes; 1996 Feb; 10(1):51-5. PubMed ID: 8684376.
    Abstract:
    We describe here the use of PCR-generated templates incorporating T3 polymerase sites in order to prepare digoxigenin (DIG)-labelled cRNA probes against any gene of known sequence. This method was applied to the preparation of probes specific for chicken glyceraldehyde-3-phosphate dehydrogenase messenger RNAs and we demonstrate that such probes can be used for in situ hybridization (ISH). This technique therefore represents a rapid and convenient means to prepare DIG-labelled cRNA probes for use in a non-radioactive ISH. It adds speed and convenience of probe preparation to the previously described advantages of non-radioactive detection techniques.
    [Abstract] [Full Text] [Related] [New Search]