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Title: An improved procedure for in situ RTPCR. Author: Volpi L, Doneda L, Grimoldi MG, Pollina E, Larizza L. Journal: Mol Cell Probes; 1996 Feb; 10(1):63-6. PubMed ID: 8684378. Abstract: Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.[Abstract] [Full Text] [Related] [New Search]