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  • Title: [An effective method of purifying recombinant human tumor necrosis factor alpha].
    Author: Tikhonov RV, Iakimov SA, Korobko VG, Vul'fson AN.
    Journal: Bioorg Khim; 1996 Mar; 22(3):163-7. PubMed ID: 8687507.
    Abstract:
    An efficient and productive isolation method for human recombinant tumor necrosis factor alpha from Escherichia coli cells was developed. The method includes a membrane filtration step, two steps of ion-exchange chromatography, and gel filtration on a Sephadex G-25 column. The target product was obtained with approximately 50% total yield and greater than 95% purity according to PAGE and HPLC.
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