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  • Title: [Determination of free d-biotin--comparison of instrumental and non-instrumental methods of analysis].
    Author: Pavlova IS, Liubavina IA, Lukin IuV, Tsibul'skaia MI, Korolev PN.
    Journal: Bioorg Khim; 1996 Mar; 22(3):223-7. PubMed ID: 8687511.
    Abstract:
    Simple methods for determining free d-biotin were developed based on the latex agglutination reaction inhibition and competitive ELISA. Biotin-binding protein Str used as a biospecific marker was immobilized on the surface of colored polyacrolein latex particles or a nitrocellulose membrane. The sensitivity of both methods of the free d-biotin assay, which take 1.5 h, is 1.0-3.0 ng/ml.
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