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  • Title: Replication of bovine respiratory syncytial virus in ovine peripheral blood lymphocytes and monocytes in vitro.
    Author: Sharma AK, Woldehiwet Z.
    Journal: Vet Microbiol; 1996 Jan; 48(1-2):125-34. PubMed ID: 8701568.
    Abstract:
    Adherent and non-adherent mononuclear cells obtained from the peripheral blood of normal lambs supported the replication of bovine respiratory syncytial virus in vitro. Sequential treatment of monocytes with phorbol ester acetate (PMA) enhanced their ability to support viral replication. After exposure in vitro for 24 h, viral antigens were present in 47 +/- 4.5% of monocytes and 32 +/- 3% of lymphocytes. Treatment of monocytes with PMA resulted in the increase of the proportion of cells expressing viral antigen and in the titre of infectious virus.
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