These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Simple high-performance liquid chromatographic method for the quantitation of 5-fluorouracil in human plasma.
    Author: Compagnon P, Thiberville L, Moore N, Thuillez C, Lacroix C.
    Journal: J Chromatogr B Biomed Appl; 1996 Mar 03; 677(2):380-3. PubMed ID: 8704945.
    Abstract:
    A simple, rapid, specific and sensitive high-performance liquid chromatography method has been developed for quantitation of 5-fluorouracil (5-FU) in human plasma. The method involves deproteinization of a small sample volume of plasma (150 microliters) followed by HPLC on a cation-exchange resin column, Aminex HPX-87H (300 x 7.8 mm I.D.), preceded by a similar guard cartridge with UV detection at 265 nm. This method allows a good separation of 5-FU with a retention time of 24 min and a detection limit at 25 ng/ml. The calibration curve was linear from 25 to 2000 ng/ml. The coefficient of variation was < or = 4.4% for within-day reproducibility and < or = 9.5% for day-to-day reproducibility.
    [Abstract] [Full Text] [Related] [New Search]