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  • Title: Development of a placental blood circulation in rat embryos in vitro.
    Author: New DA, Coppola PT.
    Journal: J Embryol Exp Morphol; 1977 Feb; 37(1):227-35. PubMed ID: 870593.
    Abstract:
    Rat embryos explanted with their membranes at head-fold stage (9 1/2 days gestation) formed an allantoic placenta which enlarged in culture and developed a foetal blood circulation. Embryos explanted at early somite stages (10 1/2 days) also formed a growing allantoic placenta but only after removal of most of the ectoplacental trophoblast. Assays of total protein in the embryo and placenta suggested that, in the absence of a maternal blood circulation to the placenta, embryo and placenta compete for the respiratory and nutritional resources obtained through the yolk-sac.
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