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  • Title: Structural analysis of the O-antigenic polysaccharide from the enteropathogenic Escherichia coli O125.
    Author: Kjellberg A, Urbina F, Weintraub A, Widmalm G.
    Journal: Eur J Biochem; 1996 Jul 15; 239(2):532-8. PubMed ID: 8706764.
    Abstract:
    The structure of the polysaccharide part of the lipopolysaccharide obtained from the enteropathogenic Escherichia coli O125 has been investigated. Methylation analysis, 1H-NMR spectroscopy and 13C-NMR spectroscopy revealed that the polysaccharide is composed of repeating hexasaccharide units. Smith degradation of the native O-polysaccharide resulted in a polysaccharide with four sugar residues in the repeating unit. Information on the sequence of the native O-polysaccharide and the Smith-degraded product was obtained by two-dimensional techniques, namely heteronuclear-multiple-bond-connectivity and NOESY experiments. The structure of the repeating unit of the O-polysaccharide of E. coli strain O125, which has two adjacent branch-point residues, is [sequence: see text].
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