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  • Title: The role of pregnenolone-metabolizing enzymes in the regulation of oestradiol biosynthesis during development of the first wave dominant follicle in the cow.
    Author: Carrière PD, Harvey D, Cooke GM.
    Journal: J Endocrinol; 1996 May; 149(2):233-42. PubMed ID: 8708534.
    Abstract:
    During the luteal phase in the cow, a first-wave dominant follicle grows to reach ovulatory size, but then ceases to grow, becomes no longer dominant and enters a phase of slow regression. During this growth transition, the concentration of oestradiol has been shown to decrease in follicular fluid. The objective of this study was to determine if follicular fluid oestradiol concentrations are regulated by the activity of three major steroidogenic enzymes, namely P450-aromatase (P450-arom), 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) and 17 alpha-hydroxylase C-17,20 lyase cytochrome P450 enzyme (P450-17 alpha) measured in granulosa and theca cells isolated from individual first-wave dominant follicles. Follicle growth and state of dominance was assessed by ultrasonography and follicles were classified as growing-dominant (GD, n = 6), non-growing-dominant (NGD, n = 8) or non-growing-non-dominant (NGD, n = 6). Mean follicular fluid concentrations of oestradiol were higher in GD than in NGD or NGND follicles (511 +/- 98 versus 136 +/- 16 and 20 +/- 11 nmol/l respectively). Oestradiol was not correlated with P450-arom in any of the three groups. In GD follicles, oestradiol was positively correlated with pregnenolone concentration but neither was correlated with granulosa or theca 3 beta-HSD activity or with theca P450-17 alpha activity. In NGD follicles, oestradiol was negatively correlated with theca 3 beta-HSD activity and pregnenolone was negatively correlated with granulosa 3 beta-HSD activity. In NGND follicles, oestradiol was positively correlated, and pregnenolone was negatively correlated with theca 3 beta-HSD and P450-17 alpha activities. These studies demonstrated that pregnenolone supply is the principal regulating factor of oestradiol output during follicle dominance and during the loss of dominance but that the levels of P450-17 alpha and 3 beta-HSD activity become rate-limiting when the follicle is no longer dominant.
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