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  • Title: TolC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli.
    Author: Foreman DT, Martinez Y, Coombs G, Torres A, Kupersztoch YM.
    Journal: Mol Microbiol; 1995 Oct; 18(2):237-45. PubMed ID: 8709843.
    Abstract:
    STB secretion-deficient mutants were isolated using the synthetic transposon Tn beta laM. Cultures were plated using a double-membrane system of cellulose acetate and nitrocellulose placed on Luria agar plates containing carbenicillin. The STB bound to the underlying nitrocellulose membrane was detected with anti-STB antibodies. The altered genes of two STB secretion-deficient mutants were identified by conjugation and complementation as tolC and dsbA. In cultures of well-characterized dsbA and tolC mutants, STB was absent from the culture supernatant. The role of TolC and DsbA in the secretion of peptides is discussed.
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