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  • Title: [Whole cell recording of sodium, potassium and calcium currents of cultured neonatal rat sympathetic neurons].
    Author: He XP, Liu QS, Xie ZP, Liu CG.
    Journal: Sheng Li Xue Bao; 1995 Oct; 47(5):441-7. PubMed ID: 8711507.
    Abstract:
    Na, K and Ca currents and other electrophysiological characteristics of cultured neonatal rat superior cervical sympathetic neurons were studied using whole cell clamp technique. The mean passive and active membrane properties measured are as follows: resting membrane potential, -51 +/- 6 mV; input resistance, 1432 +/- 389 M omega; time constant, 130 +/- 32 ms; amplitude of action potential, 96 +/- 10 mV; overshoot, 42 +/- 6 mV. Na, K and Ca currents were isolated upon pharmacological manipulations. The predominant type of K current was a noninactivating delayed rectifier. Voltage-clamp studies also showed the presence of a high voltage-activated sustained inward Ca current, while low voltage could not elicit any transient Ca current.
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