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  • Title: [Aspects of the biosynthesis of actinomycin C].
    Author: Orlova TI, Alekhova TA, Silaev AB.
    Journal: Antibiotiki; 1979 Apr; 24(4):246-50. PubMed ID: 87146.
    Abstract:
    The protoplasts of Actinomyces sp. 26--115 producing actinomycin C were obtained by the action of lysozyme on the mycelial paste of a 48-hour microbial culture. The protoplast capacity for synthesizing actinomycin was decreased as compared to that of the intact mycelium. The transport of L-isoleucine, a precursor of actinomycin C biosynthesis in the protoplasts also decreased but this could not be the only cause of the decrease in the actinomycin biosynthesis capacity. The biosynthesis of actinomycin C by the protoplasts of Actinomycin sp. 26--115 did not require galactose and was not inhibited by glucose and exogenic actinomycin.
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