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  • Title: Cloning and sequence analysis of a putative transcription factor (MTF1) gene from Mucor circinelloides.
    Author: Mukhtar M, Käufer NF, Logan DA.
    Journal: Biochem Mol Biol Int; 1996 Apr; 38(4):791-9. PubMed ID: 8728109.
    Abstract:
    We describe here the fortuitous cloning of a putative transcription factor gene (MTF1) from the dimorphic fungus Mucor circinelloides. Sequence analysis of MTF1 revealed an open reading frame (ORF) of 1059 nucleotides encoding a protein of M(r) 39601. The deduced amino acid sequence from the ORF imparts two glutamine-rich stretches which are homologous to a number of transcription factors characterized previously from various organisms. A Southern blot analysis of Mucor genomic DNA digested with different restriction endonucleases and probed with the 1.9 kb EcoR1 fragment of the putative transcription factor gene shows a single copy number of the the gene. Northern analysis during morphogenetic changes in Mucor suggested constitutive expression of the gene.
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