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  • Title: Recovery of human basophils after FMLP-stimulated secretion.
    Author: Dvorak AM, Warner JA, Fox P, Lichtenstein LM, Macglashan DW.
    Journal: Clin Exp Allergy; 1996 Mar; 26(3):281-94. PubMed ID: 8729665.
    Abstract:
    BACKGROUND: Basophils are circulating, secretory granulocytes that are generally considered to be end-stage cells. In one species of guinea-pigs, basophilic leucocytes have been shown to recover from stimulated secretion in short-term cultures. Similar studies have not been done using human basophils. OBJECTIVE: The purpose of this study was to examine human basophils in short-term recovery intervals following stimulation of secretion to determine whether visual evidence of recovery occurred. METHODS: We examined the ultrastructural morphology of early recovery (10 min-6 h) of human basophils following secretion stimulated by formyl-methionyl-leucyl-phenyl-alanine (FMLP). A combined technique for electron microscopy consisted of post-fixation exposure to cationized ferritin and reduced osmium, providing maximum quality images and allowing identification of intracellular spaces/organelles that opened to the cell surface, often out of the plane of section. RESULTS: The ultrastructural evaluation revealed that control basophils (0 time-6 h) did not undergo regulated secretion or develop the morphologies associated with recovery following secretion. FMLP-stimulated basophils underwent an overlapping continuum of piecemeal degranulation-->anaphylactic degranulation (0 time-1 min), producing vesicle- and granule-free, completely degranulated, viable, mature basophils with polylobed nuclei. The early recovery period (10 min-6 h) following FMLP stimulation was characterized by reconstitution of granules. Morphological mechanisms for granule reconstitution included a mixture of conservation, condensation, and synthetic events. CONCLUSION: Human basophils, like guinea pig basophils, have the potential to recover from regulated secretion.
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