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Pubmed for Handhelds

PUBMED FOR HANDHELDS

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  • Title: A microscope stage sample holder for microfluorometric measurements of biological specimen in the range of temperature between 3.5 to 300 K.
    Author: Tiffe HW.
    Journal: Histochemistry; 1977 May 20; 52(2):171-7. PubMed ID: 873814.
    Abstract:
    A cryostat was constructed, which permits measurements of the intracellular fluorescence within a range of temperature between 3.5 to 300 K. The cooling unit operates in accordance with the "Continuous Flow Principle" and allows the application of objectives up to a numerical aperture of 0.6. It results from measurement of BAO Feulgen stained nuclear DNA, that a decrease of fluorescence intensity is caused by two different mechanisms: (1) There is a highly temperature dependent effect, originating from phenomena of solid physics, and (2) a second effect, which is almost temperature independent, and can be explained as a photochemical reaction.
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