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  • Title: Internalization of wheat germ agglutinin (WGA) by rat pancreatic cells in vivo and in vitro.
    Author: Wirth C, Schwuchow J, Jonas L.
    Journal: Acta Histochem; 1996 Apr; 98(2):165-72. PubMed ID: 8739301.
    Abstract:
    The uptake of the lectin wheat germ agglutinin (WGA) by the rat pancreas was studied after intraperitoneal injection of FITC-labelled WGA. Strong fluorescence was seen in the interstitial space of the pancreatic parenchyma and on the basolateral surface of acinar cells after one h. Two or four h after injection there was also a fluorescence inside the acinar cells. After intraparenchymal injection of colloidal gold-labelled WGA into the pancreatic gland in situ or in vitro incubation of isolated rat pancreatic acinar cells with WGA-gold, an internalization of the lectin by receptor mediated endocytosis was observed electron microscopically. Clathrin-coated pits or vesicles could not be observed. The results suggest a direct uptake of WGA from the peritoneum into the gland by diffusion and its binding as well as internalization by the pancreatic acinar cells.
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