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  • Title: Evidence for hydroxyl radical production by human neutrophils.
    Author: Tauber AI, Babior BM.
    Journal: J Clin Invest; 1977 Aug; 60(2):374-9. PubMed ID: 874097.
    Abstract:
    The possibility that neutrophils produce the hydroxyl radical (OH-) was studied by examining the ability of these cells to support the release of ethylene from methional, a reaction in which it has been shown that OH-, but not O2- or H2O2, may serve as the oxidizing agent. When neutrophils were exposed to opsonized zymosan in the presence of 0.35 mM methional, ethylene was released in quantities amounting to 44.6+/-3.6 pmol/10(6) cells/40 min. Ethylene production required the presence of neutrophils, opsonized zymosan, and methional, indicating that it was formed from methional by stimulated but not resting neutrophils. Ethylene was not produced by zymosan-treated cells from patients with chronic granulomatous disease, confirming the requirement for respiratory burst activity in this process. Ethylene production was suppressed by benzoic acid, an OH- scavenger. Superoxide dismutase (3 microgram/ml) reduced ethylene production to 21% of control levels, but catalase had no significant effect in this system. These findings indicate that stimulated neutrophils produce a highly reactive oxidizing radical, possibly OH-, which releases ethylene from methional, and that the O2-generated during the respiratory burst is involved in the production of this reactive species.
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