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  • Title: An immunohistochemical study of the endocrine pancreas of ducks.
    Author: Lucini C, Castaldo L, Lai O.
    Journal: Eur J Histochem; 1996; 40(1):45-52. PubMed ID: 8741099.
    Abstract:
    Pancreatic tissue specimens of domestic ducks were fixed in PAF, paraformaldehyde and Bouin's fluid. Sections were immunohistochemically stained with antisera against insulin, glucagon, somatostatin and 5-hydroxytryptamine (5-HT). Immunoreactive cells were detected in both exocrine and endocrine pancreas. The endocrine cells were grouped into three islet types: A, B and mixed islets. A islets comprised glucagon, somatostatin and 5-HT immunoreactive cells. B islets showed insulin, somatostatin and 5-HT immuno-reactive cells. Mixed islets were composed of all four immunoreactive cytotypes. Using a double immunofluorescent technique, it was observed that 5-HT immunoreactivity was present in some of the glucagon and in some of the insulin immunoreactive cells. B islets were observed throughout the pancreas. A and mixed islets were more concentrated in the splenic lobe, and decreased in number in the other lobes. These results were compared with those obtained in other avian species.
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