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Title: Meiotic spindle organization in fertilized Drosophila oocyte: presence of centrosomal components in the meiotic apparatus. Author: Riparbelli MG, Callaini G. Journal: J Cell Sci; 1996 May; 109 ( Pt 5)():911-8. PubMed ID: 8743938. Abstract: We examined spindle reorganization during the completion of meiosis in fertilized and unfertilized oocytes of Drosophila using indirect immunofluorescence and laser scanning confocal microscopy. The results defined a complex pathway of spindle assembly during resumption of meiosis, and revealed a transient array of microtubules radiating from the equatorial region of the spindle towards discrete foci in the egg cortex. A monastral array of microtubules was observed between twin metaphase II spindles in fertilized and unfertilized eggs. The microtubules originated from disk-shaped material stained with Rb188 antibody specific for an antigen associated with the centrosome of Drosophila embryos. The Drosophila egg, therefore, contains a maternal pool of centrosomal components undetectable in mature inactivated oocytes. These components nucleate microtubules in a monastral array after activation, but are unable to organize bipolar spindles.[Abstract] [Full Text] [Related] [New Search]