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  • Title: [Detection of anti-Treponema pallidum antibodies with colloidal gold immunoprobes].
    Author: Burgos JD, Mendoza M, Vinasco J, Hernández R.
    Journal: Enferm Infecc Microbiol Clin; 1996 May; 14(5):304-7. PubMed ID: 8744370.
    Abstract:
    BACKGROUND: The aim of this study was to evaluate the usefulness of colloidal gold probes widely used in histochemistry and electronic microscopy for the serologic diagnosis of syphilis. METHODS: One hundred eighty-nine sera which had been submitted to the traditional fluorescent trigenemic absorption (FTA-ABS) test and had later been tested with the new colloidal gold probe method (IAT-ABS). RESULTS: Out of a total of 103 positive samples by FTA-ABS all were positive when tested by gold immunoprobes (sensitivity 100%). Eighty-six samples which were negative by immunofluorescence were all negative by IAT-ABS (specificity 100%). CONCLUSIONS: The new method of colloidal gold probes with the current microscopy is useful for confirming serologic diagnosis of syphilis.
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