These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Expression of aberrantly spliced growth hormone receptor mRNA in the sex-linked dwarf chicken, Gifu 20.
    Author: Tanaka M, Hayashida Y, Wakita M, Hoshino S, Nakashima K.
    Journal: Growth Regul; 1995 Dec; 5(4):218-23. PubMed ID: 8745148.
    Abstract:
    The structure and expression of the growth hormone receptor (GHR) gene have been studied in a sex-linked dwarf (SLD) chicken strain, Gifu 20. No gross structural change in the receptor gene was observed by Southern blot analysis, however, a transcript of 5.5 killobases (kb), which was approximately 1 kb larger than the usual chicken full length-GHR mRNA was detected in the dwarf chicken by Northern blot analysis. GHR cDNA was obtained from dwarf chicken liver RNA by reverse transcription-polymerase chain reaction, and nucleotide sequence analysis revealed that it contained an intron sequence of the GHR gene. The unspliced intron showed a single point mutation at the donor site from GT to GC, and an aberrant stop codon was generated in the extracellular domain-coding region. Thus, the mutation resulting in an inappropriate splicing of the GHR gene transcript is considered to be the cause of this dwarf chicken.
    [Abstract] [Full Text] [Related] [New Search]