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  • Title: Localization of ribosomal genes in human spermatogonia by fluorescent in situ hybridization and confocal microscopy.
    Author: Lévy N, Navarro A, Depetris D, Moretti C, Stahl A.
    Journal: Cytogenet Cell Genet; 1996; 73(4):290-4. PubMed ID: 8751378.
    Abstract:
    The distribution of ribosomal RNA genes in nucleoli is still a matter of controversy. We have investigated the nucleolus of human type A spermatogonia, which displays a single, large fibrillar center. Silver-staining was used to localize the fibrillar center by light microscopy. Fluorescent in situ hybridization was performed on the same cell, using a 5.8-kb probe specific for a transcribed region of the ribosomal genes. The fluorescent area exactly corresponded to the silver-stained area. The three-dimensional distribution of rDNA was studied in horizontal and orthogonal sections using a confocal laser scanning microscope. The presence of the fluorescent signal throughout the whole silver-stained structure demonstrated that the fibrillar center, and at least a part of the dense fibrillar component, contained most of the rDNA.
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