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  • Title: A flow cytometric study on giant cell tumor of bone.
    Author: Guo W, Ren X, Feng C.
    Journal: Chin Med J (Engl); 1996 Feb; 109(2):134-7. PubMed ID: 8758338.
    Abstract:
    OBJECTIVE: Giant cell tumor of bone (GCT) is a comparatively common bone tumor in Chinese. Though GCT is generally considered as a benign tumor, it can pursue an aggressive course. The present study attempts to clarify whether the flow cytometric study can be an useful tool for prognosis of GCT patients. METHODS: DNA content and cell cycle studies on 96 pathologically confirmed GCT specimens and anti-GCT monoclonal antibody (GCF-5 McAb) labeling study on 30 GCTs were made by flow cytometry. RESULTS: 29 GCTs (30.2%) exhibited aneuploid and 67 (69.8%) diploid. Of 71 primary tumors, 16 (22.5%) exhibited aneuploid. The DNA index was 0.97+0.15, and the average S, G2/M phase cells 15.23+6.15%. Of 25 recurrent tumors, 13 (52%) were aneuploid, with an average DI of 1.08+0.17. The average S, G2/M phase cells was 19.56+7.12%. Between the primary and recurrent tumor groups, there was significant statistical difference both in S, G2/M phase cell percentage (P < 0.05) and DNA content (P < 0.01). There was no significant difference between Jaffe's grade I and grade II groups in DNA content or in S, G2/M phase cell percentage. GCF-5 McAb could combine with almost all cells of S, G2/M phase and part of the cells in G0/G1 phase. Significant relationship existed (r=0.468, P < 0.05) between the percentages of S, G2/M phase cells and GCF-5 positive cells. Between the primary and the recurrent tumor groups, there was a significant difference in the percentage of both S, G2/M phase cells and GCF-5 positive cells. CONCLUSION: Apparently flow cytometric study is of value in the evaluation of the biological behavior of GCT as well as the prognosis of the patients.
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