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  • Title: [Modulation of bcl-2 antisense RNA on programmed cell death of leukemic cell line].
    Author: Chen X, Wang W, Huang G.
    Journal: Zhonghua Yi Xue Za Zhi; 1996 Feb; 76(2):112-5. PubMed ID: 8758442.
    Abstract:
    OBJECTIVE: To investigate modulation of decrease of intrinsic bcl-2 protein levels on programmed cell death of leukemia cells. METHOD: Gene transfection procedure was applied to observe the effect of antisense RNA-mediated suppression of bcl-2 gene expression on programmed cell death of human T-lymphocytic leukemia cell line CEM. RESULTS: Temporary expression of antisense bcl-2 gene could effectively reduce levels of intrinsic bcl-2 protein of CEM cells and render it more sensitive to etoposide-induced cytotoxicity. Moreover, a great deal of apoptotic bodies and ladder DNA was always produced during etoposide-mediated killing of CEM and when CEM expressing bcl-2 antisense RNA served as target cells in particular, the amount of ladder DNA increased to around 40%. CONCLUSION: Programmed cell death is one of the mechanisms by which etoposide kills leukemic cells and is modulated by cellular intrinsic bcl-2 protein.
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