These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: [Construction of shuttle vector containing delta-endotoxin gene of Bacillus thuringiensis].
    Author: Sun L, Ba F, Liang P, Tian Y, Mei R, Mang K.
    Journal: Wei Sheng Wu Xue Bao; 1996 Feb; 36(1):69-72. PubMed ID: 8762474.
    Abstract:
    The Bacillus thuringiensis toxin gene CryIA(c) was inserted into the shuttle vector pBE-2 to construct pAMY for expressing the B.t. gene in both Gram-negative and -positive bacterial systems. pAMY was introduced into wild type Bacillus cereus, B.brevis and B.subtilis by electroporation. Transformants containing delta-endotoxin gene produced proteins reacted with B.t. crystal protein antibody. Upon biological toxicity tests, the transformants gave a mortality of 100% against Ostrinia furnacilis, 58.8% against Heliothis armigera and 100% against Heliothis assulta. The ability of promoting plant growth of the original strains is retained.
    [Abstract] [Full Text] [Related] [New Search]