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  • Title: Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.
    Author: Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L.
    Journal: J Bacteriol; 1996 Jul; 178(14):4313-8. PubMed ID: 8763965.
    Abstract:
    The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.
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