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  • Title: [The ultrastructural characteristics of the epithelial cells in the frog bladder under the action of vasopressin and in a vasopressin-independent increase in permeability for water].
    Author: Komissarchik IaIu, Natochin IuV, Shakhmatova EI, Brudnaia MS, Snigirevskaia ES, Korolev EV, Parnova RG.
    Journal: Tsitologiia; 1996; 38(1):14-21. PubMed ID: 8768547.
    Abstract:
    In experiments on isolated frog urinary bladders it has been found that the low basal level of water permeability in the absence of arginine-vasopressin (AVP) could be significantly increased when the serosal solution was changed several times every 15 min for a fresh Ringer solution. The electron microscopic study of these cells by the freeze-fracture technique showed that the enhancement of water permeability by AVP-independent manner was related to the appearance of intramembranous particle aggregates in luminal membrane of granular cell, that are usually observed only under the action of AVP. The immunocytochemical experiments with monoclonal antibodies against actin revealed the similarity in intracellular actin distribution under the action of AVP and AVP-independent increase of water permeability.
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