These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Intracellular calcium stores in isolated tracheal smooth muscle cells.
    Author: Sims SM, Jiao Y, Zheng ZG.
    Journal: Am J Physiol; 1996 Aug; 271(2 Pt 1):L300-9. PubMed ID: 8770069.
    Abstract:
    We investigated the effects of acetylcholine (ACh) and histamine on intracellular calcium concentration ([Ca2+]i) and contraction of freshly isolated guinea pig tracheal smooth muscle cells. Previous electrophysiological studies revealed that agonists elicit cation and Cl- currents, but a role for Ca2+ in mediating these effects remains unresolved. Here we characterize agonist-induced changes of [Ca2+]i, using fura 2, and examine the contribution of the sarcoplasmic reticulum (SR) to regulation of [Ca2+]i. We provide evidence that the rise of [Ca2+]i and the contraction elicited by ACh or histamine are largely due to release of Ca2+ from stores. Agonists elicited Ca2+ transients in Ca(2+)-free solution with 0.5 mM ethylene glycol-bis (beta-aminoethyl ether)-N, N, N',N'-tetraacetic acid (EGTA), whereas prolonged exposure to Ca(2+)-free solution diminished the rise of [Ca2+]i. In addition, blockade of SR Ca(2+)-adenosinetriphosphatase (ATPase) by cyclopiazonic acid (CPA) or thapsigargin caused elevation of [Ca2+]i and reduction of ACh-evoked increase of [Ca2+]i. In many cells, [Ca2+]i fell below baseline (undershoot) after ACh or caffeine. CPA abolished this undershoot and reduced the rate of recovery of [Ca2+]i to basal levels. Furthermore, oscillations of [Ca2+]i were elicited in the presence or absence of extracellular Ca2+, and these too were reversibly abolished by CPA. Our results provide evidence that Ca2+ stores play a significant role in agonist-mediated increase of [Ca2+]i in tracheal muscle and that the SR contributes to the restoration of basal Ca2+ levels.
    [Abstract] [Full Text] [Related] [New Search]