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  • Title: Rapid purification of two thermophilic proteinases using dye-ligand chromatography.
    Author: Cowan DA, Daniel RM.
    Journal: J Biochem Biophys Methods; 1996 Apr; 32(1):1-6. PubMed ID: 8773542.
    Abstract:
    Dye-ligand chromatography has been used successfully for the purification of extracellular thermostable proteinases from thermophilic Bacillus and Thermus cultures. Single-step purification factors of up to 115-fold (for Thermus protease) and 2195-fold (for Bacillus protease) were obtained. Elution studies suggested that the mode of binding involved the enzyme active sites. The method was readily scalable to 600 1 volume.
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