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  • Title: Azurocidin, a natural antibiotic from human neutrophils: expression, antimicrobial activity, and secretion.
    Author: Almeida RP, Vanet A, Witko-Sarsat V, Melchior M, McCabe D, Gabay JE.
    Journal: Protein Expr Purif; 1996 Jun; 7(4):355-66. PubMed ID: 8776752.
    Abstract:
    The azurophil granules of human PMN contain four antibiotic proteins, the serprocidins, which have extensive homology to one another and to serine proteases. Azurocidin, a member of this family, is a 29-kDa glycoprotein with broad spectrum antimicrobial activity and chemotactic activity toward monocytes. Insect cells transfected with a baculovirus vector carrying azurocidin cDNA produced a recombinant azurocidin protein. We purified the recombinant azurocidin protein from the culture medium of the infected cells and showed that it retained the antimicrobial activity of the native neutrophil-derived molecule. In addition, we present evidence that a 49-amino-acid region of the recombinant azurocidin protein is required for its secretion from insect cells.
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